ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (12): 2206-2217.doi: 10.11843/j.issn.0366-6964.2015.12.012

Previous Articles     Next Articles

The Effect of Different Levels of Lactoferrin on the Growth Performance,Small Intestinal Morphology and Body Resistance to Disease of Diansa Weaning Piglets

AN Qing-cong#,XU Na-na#,ZHANG Chun-yong,PAN Hong-bin,LI Mei-quan,CHEN Ke-lin,GUO Rong-fu*   

  1. (Key Laboratory of Animal Nutrition and Feed of Yunnan Province,College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)
  • Received:2015-02-05 Online:2015-12-23 Published:2015-12-23

Abstract:

This experiment was conducted to investigate the effects of different levels of lactoferrin(LF) on growth performance,intestinal protection and the body resistance to disease of Diansa weaning piglets.Two hundred and forty healthy Diansa weaning piglets,(28±2) days of age with(6.56±0.75) kg of average body weight,were allocated randomly to 5 groups,48 for each group,and each had 6 replicates with 8 piglets per replicate.They were fed with basal diet(control group),basal diet + 125 mg•kg-1 LF,basal diet + 250 mg•kg-1 LF,basal diet + 500 mg•kg-1 LF and antibiotics positive group(50 mg•kg-1 terramycin),respectively.The trial period lasted 42 d.At the end,24 piglets,with 4 piglets in each replicate per each group,were selected,and the test piglets were collected blood from precava,then serum were separated.The IgG and NO content,LDH,NOS and LSZ activity and TIBC of serum were detected.Six piglets,1 piglet in each replicate per each group,were slaughtered.The heart,liver and spleen were collected to detect Hepcidin and β defense 1(pBD-1),β defense 2(pBD-2) and β defense 3(pBD-3) genes mRNA expression.The results showed that:1) The final weight,ADG and ADFI of 250 mg•kg-1 LF group were significantly higher than those of control group and terramycin group(P<0.05),feed conversion and diarrhea rate were significantly lower than those of control group and terramycin group(P<0.05).2) IgG levels,NO content of 250 mg•kg-1 LF group were significantly higher than those of control group(P<0.05),TIBC of 250 mg•kg-1 LF group were extremely significantly higher than those of control group and terramycin group(P<0.01).V/C of duodenum,villus height and V/C of jejunum,villus height and V/C of ileum in 250 mg•kg-1 LF group were extremely significantly higher than those in control group and terramycin group(P<0.01),crypt depth of ileum in 250 mg•kg-1 LF group were extremely significantly lower than those in control group(P<0.01).Results showed that diarrhea rate,IgG,NO,LSZ,crypt depth of duodenum,V/C of duodenum,villus height of jejunum,crypt depth of jejunum,V/C of jejunum increased with a quadratic curve by the LF adding levels(P<0.05);3) Hepcidin mRNA expression in heart and liver of piglets in 250 mg•kg-1 LF group were extremely significantly higher than those of control group and terramycin group(P<0.01).Hepcidin mRNA expression in liver in 125 mg•kg-1 LF group were significantly higher than those of control group and terramycin group(P<0.05).The Hepcidin mRNA expression in liver was extremely significantly higher than that in the heart and spleen(P<0.01).The pBD-1 mRNA expression in the heart and spleen in 250 mg•kg-1 LF group were extremely significantly higher than those of the other groups(P<0.01).The pBD-2 mRNA expression in liver was significantly or extremely significant higher than those in heart and the spleen(P<0.05,P<0.01).When the adding level was 250 mg•kg-1,the pBD-3 mRNA expression in heart and spleen were extremely significantly higher than the other groups(P<0.01).Results suggest that adding LF in the diet can obviously improve the growth performance,serum immunological parameters and small intestinal morphology of Diansa weaning piglets,and enhance piglets disease resistance through inducing genes expression of HepcidinpBD-1,pBD-2,pBD-3 in heart,liver and spleen of Diansa weaning piglets.In this experimental conditions,based on quadratic curve results of growth performance,serum immunological and small intestinal morphology parameters,the adequate amount of LF in Diansa weaning piglets diet was 250-300 mg•kg-1.

CLC Number: